Description
Accession
F6V5Q9
Source
DNA sequence encoding Rhesus macaque CD5 including a C-His-Avi tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Rhesus macaque CD5 is a protein consisting of amino acid residues [Arg25-Pro375] ,The protein has a predicted MW of 41.7 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Rhesus macaque CD5 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Rhesus macaque CD5 Protein Tris-Bis PAGE
- Rhesus macaque CD5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Rhesus macaque CD5 Protein SEC-HPLC
- The purity of Rhesus macaque CD5 is greater than 95% as determined by SEC-HPLC.
Rhesus macaque CD5 Protein ELISA Data
- Immobilized Rhesus macaque CD5, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD5 Antibody, hFc Tag with the EC50 of 8.5ng/ml determined by ELISA.
