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Pig WNT-3A Recombinant
Categories: Food grade growth factorsPig growth factors$300.00 – $1,900.00
Description
Accession
I3LBV3
Source
DNA sequence encoding Pig WNT-3A mature chain sp|RKI3LBV3|19-352AA including a C terminal human Fc tag was expressed in HEK293 Cells.
Molecular weight
Recombinant pig WNT-3A is a protein that migrates due to glycosylation as an approximately 60kDa protein under reducing conditions in SDS-PAGE.
Purity
>96%, as determined by SDS-PAGE and HPLC
Biological Activity
Activity was measured by its ability to induce Topflash reporter activity in CHO cells. The ED50 for this effect is ＜50ng/ml.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Pig WNT-3A was lyophilized from a 0.2 μm filtered solution in PBS, pH.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
