Description
Accession
NP_001139797
Source
DNA sequence encoding Mouse TIGIT including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Mouse TIGIT is a protein consisting of amino acid residues [Gly26-Thr143] ,The protein has a predicted MW of 14 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Mouse TIGIT was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Mouse TIGIT Protein Tris-Bis PAGE
- Mouse TIGIT on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Mouse TIGIT Protein SEC-HPLC
- The purity of Mouse TIGIT is greater than 95% as determined by SEC-HPLC.
Mouse TIGIT Protein SPR Data
- Mouse CD155, His Tag immobilized on CM5 Chip can bind Mouse TIGIT, His Tag with an affinity constant of 4.00 μM as determined in SPR assay (Biacore T200).
