Description
Accession
P47741
Source
DNA sequence encoding Mouse OX40/TNFRSF4/CD134 including a C-His-Avi tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Mouse OX40/TNFRSF4/CD134 is a protein consisting of amino acid residues [Val20-Pro211] ,The protein has a predicted MW of 24.2 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Mouse OX40/TNFRSF4/CD134 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
P39429
Interactor
Interactor
O88351
Interactor
O88522
Interactor
Interactor
Q02111
Interactor
Q2TBA3
Interactor
Q8CIS0
Interactor
P62991
Interactor
Q60680
Interactor
Q60855
Interactor
Q9Z0H7
Molecular function
Methods
Mouse OX40/TNFRSF4/CD134 Protein Tris-Bis PAGE
- Mouse OX40 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Mouse OX40/TNFRSF4/CD134 Protein SEC-HPLC
- The purity of Mouse OX40 is greater than 92% as determined by SEC-HPLC.
Mouse OX40/TNFRSF4/CD134 Protein ELISA Data
- Immobilized Mouse OX-40 Ligand, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Mouse OX-40, His Tag with the EC50 3.59μg/ml determined by ELISA.
