Description
Accession
P43488
Source
DNA sequence encoding Mouse OX40 Ligand/TNFSF4 including a N-hFc tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Mouse OX40 Ligand/TNFSF4 is a protein consisting of amino acid residues [Ser51-Leu198] ,The protein has a predicted MW of 42.6 kDa. Due to glycosylation, the protein migrates to 52-56 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Mouse OX40 Ligand/TNFSF4 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
P46414
Interactor
Molecular function
Methods
Mouse OX40 Ligand/TNFSF4 Protein Tris-Bis PAGE
- Mouse OX40 Ligand on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Mouse OX40 Ligand/TNFSF4 Protein ELISA Data
- Immobilized Mouse OX40 Ligand at 2μg/ml (100μl/well) on the plate. Dose response curve for Mouse OX40, His Tag with the EC50 3.59μg/ml determined by ELISA.
