Description
Accession
Q07797
Source
DNA sequence encoding Mouse LGALS3BP including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Mouse LGALS3BP is a protein consisting of amino acid residues [Thr19-Val577] ,The protein has a predicted MW of 63.64 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Mouse LGALS3BP was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Mouse LGALS3BP Protein
- Mouse LGALS3BP on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Mouse LGALS3BP Protein SEC-HPLC
- The purity of Mouse LGALS3BP is greater than 95% as determined by SEC-HPLC.
Mouse LGALS3BP Protein SPR Data
- Mouse Galectin-3, His Tag immobilized on CM5 Chip can bind Mouse LGALS3BP, His Tag with an affinity constant of 17.56 nM as determined in SPR assay (Biacore T200).
