Mouse Latent TGF beta 1/TGFB1 Protein Recombinant
Category: Receptors$232.00 – $776.00
Description
Accession
P04202
Source
DNA sequence encoding Mouse Latent TGF beta 1/TGFB1 including a N-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Mouse Latent TGF beta 1/TGFB1 is a protein consisting of amino acid residues [Leu30-Ser390] ,The protein has a predicted MW of 29.5/12.9 kDa. Due to glycosylation, the protein migrates to 40-50/14-15 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Mouse Latent TGF beta 1/TGFB1 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
P46414
Interactor
Q61810
Molecular function
Molecular function
Methods
Mouse Latent TGF beta 1/TGFB1 Protein Tris-Bis PAGE
- Mouse Latent TGF beta 1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Mouse Latent TGF beta 1/TGFB1 Protein ELISA Data
- Immobilized Mouse Latent TGF beta 1, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB6, hFc Tag with the EC50 of 0.59μg/ml determined by ELISA.
