Mouse IL-23 alpha&IL-12 beta Protein Recombinant
Category: Receptors$319.00 – $1,063.00
Description
Accession
Q9EQ14
Source
DNA sequence encoding Mouse IL-23 alpha&IL-12 beta including a N-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Mouse IL-23 alpha&IL-12 beta is a protein consisting of amino acid residues [Val22-Ala196(IL-23 alpha) & Met23-Ser335(IL-12 beta)] ,The protein has a predicted MW of 20.8 (Mouse IL-23 alpha)&35.8 (Mouse IL-12 beta) kDa. Due to glycosylation, the protein migrates to 22 (Mouse IL-23 alpha)&45-50 (Mouse IL-12 beta) kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Mouse IL-23 alpha&IL-12 beta was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Mouse IL-23 alpha&IL-12 beta Protein Tris-Bis PAGE
- Mouse IL-23 alpha&IL-12 beta on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Mouse IL-23 alpha&IL-12 beta Protein SEC-HPLC
- The purity of Mouse IL-23 alpha&IL-12 beta is greater than 95% as determined by SEC-HPLC.
Mouse IL-23 alpha&IL-12 beta Protein SPR Data
- Mouse IL-23R, His Tag immobilized on CM5 Chip can bind Mouse IL-23 alpha&IL-12 beta, His Tag with an affinity constant of 6.31 nM as determined in SPR assay (Biacore T200).
