Description
Accession
P01590
Source
DNA sequence encoding Mouse IL-2 R alpha/CD25 including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Mouse IL-2 R alpha/CD25 is a protein consisting of amino acid residues [Glu22-Lys236] ,The protein has a predicted MW of 25.76 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Mouse IL-2 R alpha/CD25 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Molecular function
Methods
Mouse IL-2 R alpha/CD25 Protein Tris-Bis PAGE
- Mouse IL-2 R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Mouse IL-2 R alpha/CD25 Protein SEC-HPLC
- The purity of Mouse IL-2 R alpha is greater than 95% as determined by SEC-HPLC.
Mouse IL-2 R alpha/CD25 Protein ELISA Data
- Immobilized Mouse IL-2 R alpha, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Mouse IL-2, His Tag with the EC50 of 95.8ng/ml determined by ELISA.
Mouse IL-2 R alpha/CD25 Protein SPR Data
- Mouse IL-2 R alpha, His Tag immobilized on CM5 Chip can bind Mouse IL-2, His Tag with an affinity constant of 30.49 nM as determined in SPR assay (Biacore T200).
