Description
Accession
Q60751
Source
DNA sequence encoding Mouse IGF1R/CD221 including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Mouse IGF1R/CD221 is a protein consisting of amino acid residues [Glu31-His936] ,The protein has a predicted MW of 80.72 kDa (alpha subunit) and 23.0 kDa (beta subunit). Due to furin cleavage and glycosylation, the protein migrates to 110-140 kDa(alpha subunit) and 50-65 kDa(beta subunit) based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Mouse IGF1R/CD221 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Mouse IGF1R/CD221 Protein Tris-Bis PAGE
- Mouse IGF1R on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
