Description
Accession
Q6SJE0
Source
DNA sequence encoding Mouse GFRAL/GFR alpha-like including a N-His-Avi tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Mouse GFRAL/GFR alpha-like is a protein consisting of amino acid residues [Gln20-Glu350] ,The protein has a predicted MW of 40.1 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Mouse GFRAL/GFR alpha-like was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Mouse GFRAL/GFR alpha-like Protein Tris-Bis PAGE
- Mouse GFRAL on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Mouse GFRAL/GFR alpha-like Protein SEC-HPLC
- The purity of Mouse GFRAL is greater than 95% as determined by SEC-HPLC.
Mouse GFRAL/GFR alpha-like Protein ELISA Data
- Immobilized Mouse GFRAL, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Mouse GDF15, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA.
Mouse GFRAL/GFR alpha-like Protein SPR Data
- Mouse GFRAL, His Tag immobilized on CM5 Chip can bind Mouse GDF15, His Tag with an affinity constant of 0.34 nM as determined in SPR assay (Biacore T200).
