Description
Accession
P49772
Source
DNA sequence encoding Mouse FLT3 Ligand including a C-hFc tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Mouse FLT3 Ligand is a protein consisting of amino acid residues [Gly27-Gln189] ,The protein has a predicted MW of 45.3 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Mouse FLT3 Ligand was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Molecular function
Methods
Mouse FLT3 Ligand Protein Tris-Bis PAGE
- Mouse FLT3 Ligand on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Mouse FLT3 Ligand Protein SEC-HPLC
- The purity of Mouse FLT3 Ligand is greater than 95% as determined by SEC-HPLC.
Mouse FLT3 Ligand Protein SPR Data
- Mouse FLT3 Ligand, hFc Tag captured on CM5 Chip via Protein A can bind Human FLT3, His Tag with an affinity constant of 13.81 nM as determined in SPR assay (Biacore T200).
