Description
Accession
Q00342
Source
DNA sequence encoding Mouse FLT3/Flk-2 including a C-hFc tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Mouse FLT3/Flk-2 is a protein consisting of amino acid residues [Asn28-Ser544] ,The protein has a predicted MW of 84.78 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Mouse FLT3/Flk-2 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Mouse FLT3/Flk-2 Protein Tris-Bis PAGE
- Mouse FLT3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Mouse FLT3/Flk-2 Protein SPR Data
- Human FLT3 Ligand, hFc Tag immobilized on CM5 Chip can bind Mouse FLT3, hFc Tag with an affinity constant of 35.07 nM as determined in SPR assay (Biacore T200).
