Description
Accession
NP_001070657
Source
DNA sequence encoding Mouse Fc gamma RIIB/CD32b including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Mouse Fc gamma RIIB/CD32b is a protein consisting of amino acid residues [Thr40-Arg217] ,The protein has a predicted MW of 21.4 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Mouse Fc gamma RIIB/CD32b was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Mouse Fc gamma RIIB/CD32b Protein Tris-Bis PAGE
- Mouse Fc gamma RIIB on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Mouse Fc gamma RIIB/CD32b Protein SEC-HPLC
- The purity of Mouse Fc gamma RIIB is greater than 95% as determined by SEC-HPLC.
Mouse Fc gamma RIIB/CD32b Protein SPR Data
- OKT3 captured on CM5 Chip via Protein A can bind Mouse Fc gamma RIIB, His Tag with an affinity constant of 0.280 μM as determined in SPR assay (Biacore T200).
