Description
Accession
P97321
Source
DNA sequence encoding Mouse FAP including a N-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Mouse FAP is a protein consisting of amino acid residues [Leu26-Asp761] ,The protein has a predicted MW of 86.4 kDa. Due to glycosylation, the protein migrates to 90-100 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Mouse FAP was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Mouse FAP Protein Tris-Bis PAGE
- Mouse FAP on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
Mouse FAP Protein SEC-HPLC
- The purity of Mouse FAP is greater than 95% as determined by SEC-HPLC.
Mouse FAP Protein ELISA Data
- Immobilized Mouse FAP, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 19.0ng/ml determined by ELISA.
