Description
Accession
Q61503
Source
DNA sequence encoding Mouse CD73/NT5E including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Mouse CD73/NT5E is a protein consisting of amino acid residues [Trp29-Lys549] ,The protein has a predicted MW of 58.6 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Mouse CD73/NT5E was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Molecular function
Methods
Mouse CD73/NT5E Protein Tris-Bis PAGE
- Mouse CD73 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Mouse CD73/NT5E Protein SEC-HPLC
- The purity of Mouse CD73 is greater than 95% as determined by SEC-HPLC.
Mouse CD73/NT5E Protein ELISA Data
- Immobilized Mouse CD73, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 31ng/ml determined by ELISA.
