Description
Accession
Q61735
Source
DNA sequence encoding Mouse CD47 including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Mouse CD47 is a protein consisting of amino acid residues [Gln19-Lys140] ,The protein has a predicted MW of 14.9 kDa. Due to glycosylation, the protein migrates to 35-50 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Mouse CD47 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Methods
Mouse CD47 Protein Tris-Bis PAGE
- Mouse CD47 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Mouse CD47 Protein SEC-HPLC
- The purity of Mouse CD47 is greater than 95% as determined by SEC-HPLC.
Mouse CD47 Protein SPR Data
- Mouse SIRP alpha, His Tag immobilized on CM5 Chip can bind Mouse CD47, His Tag with an affinity constant of 1.33 μM as determined in SPR assay (Biacore T200).
