Description
Accession
P27512
Source
DNA sequence encoding Mouse CD40/TNFRSF5 including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Mouse CD40/TNFRSF5 is a protein consisting of amino acid residues [Val24-Arg193] ,The protein has a predicted MW of 19.9 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Mouse CD40/TNFRSF5 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
B2RUR8
Interactor
O08863
Interactor
P39429
Interactor
Q60803
Biological Process
Molecular function
Methods
Mouse CD40/TNFRSF5 Protein Tris-Bis PAGE
- Mouse CD40 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Mouse CD40/TNFRSF5 Protein SEC-HPLC
- The purity of Mouse CD40 is greater than 95% as determined by SEC-HPLC.
Mouse CD40/TNFRSF5 Protein ELISA Data
- Immobilized Mouse CD40, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Mouse CD40 Ligand Trimer, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA.
