Mouse CD3E&CD3D/CD3 epsilon&CD3 delta Protein Recombinant
Category: Receptors$253.00 – $843.00
Description
Accession
P22646
Source
DNA sequence encoding Mouse CD3E&CD3D/CD3 epsilon&CD3 delta including a C-hFc tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Mouse CD3E&CD3D/CD3 epsilon&CD3 delta is a protein consisting of amino acid residues [Asp23-Asp108(CD3E) & Phe22-Ala105(CD3D)] ,The protein has a predicted MW of 36.1 kDa (CD3E) and 35.2 kDa (CD3D). Due to glycosylation,the protein migrates to 50-65 kDa and 40-50 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Mouse CD3E&CD3D/CD3 epsilon&CD3 delta was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Molecular function
Methods
Mouse CD3E&CD3D/CD3 epsilon&CD3 delta Protein Tris-Bis PAGE
- Mouse CD3E&CD3D on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Mouse CD3E&CD3D/CD3 epsilon&CD3 delta Protein SEC-HPLC
- The purity of Mouse CD3E&CD3D is greater than 95% as determined by SEC-HPLC.
