Description
Accession
Q8K094
Source
DNA sequence encoding Mouse CD155/PVR including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Mouse CD155/PVR is a protein consisting of amino acid residues [Asp29-Leu348] ,The protein has a predicted MW of 35.9 kDa. Due to glycosylation, the protein migrates to 55-75 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Mouse CD155/PVR was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Mouse CD155/PVR Protein Tris-Bis PAGE
- Mouse CD155 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Mouse CD155/PVR Protein SEC-HPLC
- The purity of Mouse CD155 is greater than 95% as determined by SEC-HPLC.
Mouse CD155/PVR Protein ELISA Data
- Immobilized Mouse CD155, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Mouse TIGIT, hFc Tag with the EC50 of 0.42μg/ml determined by ELISA.
Mouse CD155/PVR Protein SPR Data
- Mouse CD155, His Tag immobilized on CM5 Chip can bind Mouse TIGIT, His Tag with an affinity constant of 4.00 μM as determined in SPR assay (Biacore T200).
