Description
Accession
P14847
Source
DNA sequence encoding Mouse C-Reactive /CRP including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Mouse C-Reactive /CRP is a protein consisting of amino acid residues [His20-Ser225] ,The protein has a predicted MW of 24.2 kDa. Due to glycosylation, the protein migrates to 26-28 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Mouse C-Reactive /CRP was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Methods
Mouse C-Reactive Protein/CRP Protein Tris-Bis PAGE
- Mouse CRP on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Mouse C-Reactive Protein/CRP Protein SEC-HPLC
- The purity of Mouse CRP is greater than 95% as determined by SEC-HPLC.
