Description
Accession
O88472
Source
DNA sequence encoding Mouse BCMA/TNFRSF17 including a C-His-Avi tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Mouse BCMA/TNFRSF17 is a protein consisting of amino acid residues [Met1-Thr49] ,The protein has a predicted MW of 8.5 kDa. Due to glycosylation, the protein migrates to 12-16 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Mouse BCMA/TNFRSF17 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Molecular function
Methods
Mouse BCMA/TNFRSF17 Protein Tris-Bis PAGE
- Mouse BCMA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Mouse BCMA/TNFRSF17 Protein SEC-HPLC
- The purity of Mouse BCMA is greater than 95% as determined by SEC-HPLC.
Mouse BCMA/TNFRSF17 Protein ELISA Data
- Immobilized Mouse BCMA, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Mouse APRIL, hFc Tag with the EC50 of 0.63μg/ml determined by ELISA.
Mouse BCMA/TNFRSF17 Protein SPR Data
- Human BAFF, hFc Tag captured on CM5 Chip via Protein A can bind Mouse BCMA, His-Avi Tag with an affinity constant of 5.83 μM as determined in SPR assay (Biacore T200).
