Description
Accession
NP_033491
Source
DNA sequence encoding Mouse AXL including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Mouse AXL is a protein consisting of amino acid residues [His20-Trp445] ,The protein has a predicted MW of 47.8 kDa. Due to glycosylation, the protein migrates to 68-78 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Mouse AXL was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Mouse AXL Protein Tris-Bis PAGE
- Mouse AXL on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Mouse AXL Protein SEC-HPLC
- The purity of Mouse AXL is greater than 95% as determined by SEC-HPLC.
Mouse AXL Protein SPR Data
- Mouse AXL, His Tag immobilized on CM5 Chip can bind Mouse GAS6, His Tag with an affinity constant of 15.62 nM as determined in SPR assay (Biacore T200).
