Description
Accession
Q9Z0L2
Source
DNA sequence encoding Mouse ARTN including a N-hFc tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Mouse ARTN is a protein consisting of amino acid residues [Ala112-Gly224] ,The protein has a predicted MW of 39.4 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Mouse ARTN was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Molecular function
Methods
Mouse ARTN Protein Tris-Bis PAGE
- Mouse ARTN on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Mouse ARTN Protein SEC-HPLC
- The purity of Mouse ARTN is greater than 95% as determined by SEC-HPLC.
Mouse ARTN Protein ELISA Data
- Immobilized Mouse GFRA3, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Mouse ARTN, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA.
