Description
Accession
QJQ40248
Source
DNA sequence encoding Monkeypox virus E8L including a N-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Monkeypox virus E8L is a protein consisting of amino acid residues [Met1-Thr275] ,The protein has a predicted MW of 32.92 kDa. The protein migrates to 50-60 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Monkeypox virus E8L was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Monkeypox virus E8L Protein Tris-Bis PAGE
- Monkeypox virus E8L on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
