Description
Accession
P15692
Source
DNA sequence encoding Human VEGF165 including a No Tag tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human VEGF165 is a protein consisting of amino acid residues [Ala27-Arg191] ,The protein has a predicted MW of 19.2 kDa. Due to glycosylation, the protein migrates to 20-30 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human VEGF165 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Interactor
Interactor
Interactor
Interactor
Interactor
Interactor
Biological Process
Biological Process
Molecular function
Molecular function
Molecular function
Methods
Human VEGF165 Protein Tris-Bis PAGE
- Human VEGF165 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human VEGF165 Protein SEC-HPLC
- The purity of Human VEGF165 is greater than 95% as determined by SEC-HPLC.
Human VEGF165 Protein ELISA Data
- Immobilized Human VEGF165 at 1μg/ml (100μl/well) on the plate. Dose response curve for Human VEGFR2, mFc Tag with the EC50 of 74.2ng/ml determined by ELISA.
Human VEGF165 Protein
- Immobilized Human VEGF165 at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human VEGFR1, His Tag with the EC50 of 26.9ng/ml determined by ELISA.
Human VEGF165 Protein
- Immobilized Human VEGF165, No Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human Neuropilin-1, His Tag with the EC50 of 0.57μg/ml determined by ELISA.
Human VEGF165 Protein Cell Based Assay
- Human VEGF165 stimulates proliferation of human umbilical vein endothelial cells (HUVEC) with the ED50 for this effect is 2.57ng/mL.
