Description
Accession
P17948
Source
DNA sequence encoding Human VEGF R1/FLT-1 including a C-His-Avi tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human VEGF R1/FLT-1 is a protein consisting of amino acid residues [Ser27-Asn756] ,The protein has a predicted MW of 85.1 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human VEGF R1/FLT-1 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Biological Process
Biological Process
Biological Process
Molecular function
Molecular function
Molecular function
Methods
Human VEGF R1/FLT-1 Protein Tris-Bis PAGE
- Human VEGF R1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human VEGF R1/FLT-1 Protein ELISA Data
- Immobilized Human VEGF R1, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human VEGF121, His Tag with the EC50 of 36.5ng/ml determined by ELISA.
Human VEGF R1/FLT-1 Protein ELISA Data
- Immobilized Human PGF, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human VEGF R1, His Tag with the EC50 of 12.8ng/ml determined by ELISA.
