Description
Accession
Q969D9
Source
DNA sequence encoding Human TSLP (R127A, R130A) including a C-His-Avi tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human TSLP (R127A, R130A) is a protein consisting of amino acid residues [Tyr29-Gln159(R127A,R130A)] ,The protein has a predicted MW of 17.7 kDa. Due to glycosylation, the protein migrates to 25-35 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human TSLP (R127A, R130A) was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Molecular function
Methods
Human TSLP (R127A, R130A) Protein Tris-Bis PAGE
- Human TSLP (R127A, R130A) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human TSLP (R127A, R130A) Protein SEC-HPLC
- The purity of Human TSLP (R127A, R130A) is greater than 95% as determined by SEC-HPLC.
Human TSLP (R127A, R130A) Protein ELISA Data
- Immobilized Human TSLP (R127A, R130A) , His Tag at 1μg/ml (100μl/Well). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 15.5ng/ml determined by ELISA.
Human TSLP (R127A, R130A) Protein ELISA Data
- Immobilized Human TSLP (R127A,R130A) , His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-Human TSLP Ab., hFc Tag with the EC50 of 62.6ng/ml determined by ELISA.
