Description
Accession
Q9NZC2
Source
DNA sequence encoding Human TREM2 including a C-hFc tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human TREM2 is a protein consisting of amino acid residues [His19-Ser174] ,The protein has a predicted MW of 44.2 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human TREM2 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Molecular function
Methods
Human TREM2 Protein Tris-Bis PAGE
- Human TREM2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human TREM2 Protein ELISA Data
- Immobilized Human TREM2, hFc Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-TREM2 Antibody, hFc Tag with the EC50 of 25ng/ml determined by ELISA.
Human TREM2 Protein SPR Data
- Human TREM2, hFc Tag captured on CM5 Chip via Protein A can bind Human APOE3, His Tag with an affinity constant of 68.42 nM as determined in SPR assay (Biacore T200).
