Description
Accession
P02786
Source
DNA sequence encoding Human Transferrin R/CD71 including a N-His-Avi tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human Transferrin R/CD71 is a protein consisting of amino acid residues [Cys89-Phe760] ,The protein has a predicted MW of 77.9 kDa. Due to glycosylation, the protein migrates to 78-80 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human Transferrin R/CD71 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Interactor
Interactor
Interactor
Q30201
Interactor
Q8AZ57
Biological Process
Biological Process
Molecular function
Methods
Human Transferrin R/CD71 Protein Tris-Bis PAGE
- Human Transferrin R on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human Transferrin R/CD71 Protein SEC-HPLC
- The purity of Human Transferrin R is greater than 95% as determined by SEC-HPLC.
Human Transferrin R/CD71 Protein ELISA Data
- Immobilized Human Transferrin R, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-Transferrin R Antiboy, hFc Tag with the EC50 of 11.1ng/ml determined by ELISA.
Human Transferrin R/CD71 Protein SPR Data
- Human Transferrin, His Tag immobilized on CM5 Chip can bind Human Transferrin R, His Tag with an affinity constant of 27.50 nM as determined in SPR assay (Biacore T200).
