Description
Accession
NP_003801
Source
DNA sequence encoding Human TRAIL/TNFRSF10 including a No Tag tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human TRAIL/TNFRSF10 is a protein consisting of amino acid residues [Val114-Gly281] ,The protein has a predicted MW of 19.6 kDa same as Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human TRAIL/TNFRSF10 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Human TRAIL/TNFRSF10 Protein Tris-Bis PAGE
- Human TRAIL on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human TRAIL/TNFRSF10 Protein SEC-HPLC
- The purity of Human TRAIL is greater than 95% as determined by SEC-HPLC.
Human TRAIL/TNFRSF10 Protein ELISA Data
- Immobilized Human TRAIL at 2μg/ml (100μl/well) on the plate. Dose response curve for Human TRAIL R1, hFc Tag with the EC50 of 51.6ng/ml determined by ELISA.
Human TRAIL/TNFRSF10 Protein ELISA Data
- Immobilized Human TRAIL, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human TRAIL R4, His Tag with the EC50 of 0.27ug/ml determined by ELISA.
Human TRAIL/TNFRSF10 Protein SPR Data
- Human DcR1, His Tag immobilized on CM5 Chip can bind Human TRAIL, No Tag with an affinity constant of 0.57 nM as determined in SPR assay (Biacore T200).
