Description
Accession
P20333
Source
DNA sequence encoding Human TNFR2/CD120b/TNFRSF1B including a C-mFc tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human TNFR2/CD120b/TNFRSF1B is a protein consisting of amino acid residues [Leu23-Asp257] ,The protein has a predicted MW of 51.5 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human TNFR2/CD120b/TNFRSF1B was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Interactor
Q12933
Interactor
P39429
Interactor
Q9BQ06
Molecular function
Methods
Human TNFR2/CD120b/TNFRSF1B Protein Tris-Bis PAGE
- Human TNFR2Â on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
Human TNFR2/CD120b/TNFRSF1B Protein SEC-HPLC
- The purity of Human TNFR2 is greater than 95% as determined by SEC-HPLC.
Human TNFR2/CD120b/TNFRSF1B Protein ELISA Data
- Immobilized Human TNFR2 at 5μg/ml (100μl/Well). Dose response curve for Human TNF alpha, His Tag with the EC50 of 0.17μg/ml determined by ELISA.
