Description
Accession
P19438
Source
DNA sequence encoding Human TNFR1/CD120a/TNFRSF1A including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human TNFR1/CD120a/TNFRSF1A is a protein consisting of amino acid residues [Leu30-Thr211] ,The protein has a predicted MW of 23.3 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human TNFR1/CD120a/TNFRSF1A was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
O75530
Interactor
Interactor
Interactor
Interactor
Q13158
Interactor
Q15628
Interactor
Q9BQ06
Interactor
P39429
Interactor
Q12931
Interactor
Q13546
Interactor
Q15628
Interactor
Biological Process
Molecular function
Methods
Human TNFR1/CD120a/TNFRSF1A Protein Tris-Bis PAGE
- Human TNFR1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human TNFR1/CD120a/TNFRSF1A Protein SEC-HPLC
- The purity of Human TNFR1 is greater than 95% as determined by SEC-HPLC.
Human TNFR1/CD120a/TNFRSF1A Protein ELISA Data
- Immobilized Human TNFR1, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Human TNF alpha, His Tag with the EC50 of 4.8ng/ml determined by ELISA.
