Description
Accession
Q495A1
Source
DNA sequence encoding Human TIGIT including a C-hFc tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human TIGIT is a protein consisting of amino acid residues [Met22-Pro141] ,The protein has a predicted MW of 39.8 kDa. Due to glycosylation, the protein migrates to 48-50 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human TIGIT was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Human TIGIT Protein Tris-Bis PAGE
- Human TIGIT on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human TIGIT Protein SEC-HPLC
- The purity of Human TIGIT is greater than 95% as determined by SEC-HPLC.
Human TIGIT Protein ELISA Data
- Immobilized Human CD155, mFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human TIGIT, hFc Tag with the EC50 of 73.0ng/ml determined by ELISA.
Human TIGIT Protein ELISA Data
- Immobilized Biotinylated Human Nectin-2, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human TIGIT, hFc Tag with the EC50 of 2.26μg/ml determined by ELISA.
Human TIGIT Protein SPR Data
- Human TIGIT, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human Nectin-2, His-Avi Tag with an affinity constant of 1.84 μM as determined in SPR assay (Biacore T200).
