Description
Accession
P37173
Source
DNA sequence encoding Human TGF-beta RII/TGFBR2 including a C-mFc-Avi tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human TGF-beta RII/TGFBR2 is a protein consisting of amino acid residues [Ile24-Asp159] ,The protein has a predicted MW of 44 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human TGF-beta RII/TGFBR2 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Interactor
Interactor
Interactor
P10600
Interactor
Interactor
Interactor
Q03167
Biological Process
Biological Process
Molecular function
Molecular function
Methods
Human TGF-beta RII/TGFBR2 Protein Tris-Bis PAGE
- Human TGF-beta RII on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human TGF-beta RII/TGFBR2 Protein SEC-HPLC
- The purity of Human TGF-beta RII is greater than 95% as determined by SEC-HPLC.
Human TGF-beta RII/TGFBR2 Protein ELISA Data
- Immobilized Human Mature TGF beta 1, No Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 8ng/ml determined by ELISA.
Human TGF-beta RII/TGFBR2 Protein ELISA Data
- Immobilized Human TGF-beta RII, mFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Mature TGF beta 3, Avi Tag with the EC50 of 9.9ng/ml determined by ELISA.
