Description
Accession
P01135
Source
DNA sequence encoding Human TGF-alpha including a N-hFc tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human TGF-alpha is a protein consisting of amino acid residues [Val40-Ala89] ,The protein has a predicted MW of 32.9 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human TGF-alpha was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Q969F2
Molecular function
Molecular function
Methods
Human TGF-alpha Protein Tris-Bis PAGE
- Human TGF-alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human TGF-alpha Protein SEC-HPLC
- The purity of Human TGF-alpha is greater than 95% as determined by SEC-HPLC.
Human TGF-alpha Protein ELISA Data
- Immobilized Human TGF-alpha, hFc Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-TGF-alpha Antibody, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA.
Human TGF-alpha Protein SPR Data
- Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human TGF-alpha, hFc Tag with an affinity constant of 0.31 μM as determined in SPR assay (Biacore T200).
