Description
Accession
ATD50864
Source
DNA sequence encoding Human SIRP alpha V8 including a C-His-Avi tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human SIRP alpha V8 is a protein consisting of amino acid residues [Glu31-Arg369(S44L, S50T, I52T, H54R, V57A)] ,The protein has a predicted MW of 40.1 kDa. Due to glycosylation, the protein migrates to 52-68 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human SIRP alpha V8 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Human SIRP alpha V8 Protein Tris-Bis PAGE
- Human Sirpa V8 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human SIRP alpha V8 Protein SEC-HPLC
- The purity of Human Sirpa V8 is greater than 95% as determined by SEC-HPLC.
Human SIRP alpha V8 Protein ELISA Data
- Immobilized Human SIRP alpha V8, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 21.2ng/ml determined by ELISA.
