Description
Accession
AAH26692
Source
DNA sequence encoding Human SIRP alpha V2/CD172a including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human SIRP alpha V2/CD172a is a protein consisting of amino acid residues [Glu31-Arg369] ,The protein has a predicted MW of 38 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human SIRP alpha V2/CD172a was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Human SIRP alpha V2/CD172a Protein Tris-Bis PAGE
- Human SIRP alpha V2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human SIRP alpha V2/CD172a Protein SEC-HPLC
- The purity of Human SIRP alpha V2 is greater than 95% as determined by SEC-HPLC.
Human SIRP alpha V2/CD172a Protein ELISA Data
- Immobilized Human SIRP alpha V2, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 0.15μg/ml determined by ELISA.
Human SIRP alpha V2/CD172a Protein ELISA Data
- Immobilized Human SIRP alpha V2,His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-SIRP Antibody, hFc Tag with the EC50 of 10.1ng/ml determined by ELISA.
Human SIRP alpha V2/CD172a Protein Blocking Data
- Serial dilutions of Anti-SIRP alpha Antibody, hFc Tag were added into Human SIRP alpha V2, His Tag : Biotinylated Human CD47 Protein, His Tag binding reactioins.The half maximal inhibitiory concentration (IC50) is 54.14ng/ml.
Human SIRP alpha V2/CD172a Protein SPR Data
- Human SIRP alpha V2, His Tag captured on Protein A Chip via Anti-His Antibody can bind Anti-SIRP alpha Ab., hFc Tag with an affinity constant of 9.11nM as determined in SPR assay (Biacore T200).
