Description
Accession
Q96LC7
Source
DNA sequence encoding Human Siglec-10 including a C-hFc-Avi tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human Siglec-10 is a protein consisting of amino acid residues [Met17-Thr546] ,The protein has a predicted MW of 85.8 kDa. Due to glycosylation, the protein migrates to 95-110 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human Siglec-10 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Human Siglec-10 Protein Tris-Bis PAGE
- Human Siglec-10 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%
Human Siglec-10 Protein SEC-HPLC
- The purity of Human Siglec-10 is greater than 95% as determined by SEC-HPLC.
Human Siglec-10 Protein ELISA Data
- Immobilized Human Siglec-10 at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 14.4ng/ml determined by ELISA.
