Human Semaphorin 4D/SEMA4D/CD100 Protein Recombinant
Category: Receptors$202.00 – $675.00
Description
Accession
Q92854
Source
DNA sequence encoding Human Semaphorin 4D/SEMA4D/CD100 including a C-His-Avi tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human Semaphorin 4D/SEMA4D/CD100 is a protein consisting of amino acid residues [Met22-Arg734] ,The protein has a predicted MW of 82.1 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human Semaphorin 4D/SEMA4D/CD100 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Methods
Human Semaphorin 4D/SEMA4D/CD100 Protein Tris-Bis PAGE
- Human Semaphorin 4D on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human Semaphorin 4D/SEMA4D/CD100 Protein SEC-HPLC
- The purity of Human Semaphorin 4D is greater than 95% as determined by SEC-HPLC.
Human Semaphorin 4D/SEMA4D/CD100 Protein ELISA Data
- Immobilized Human Semaphorin 4D, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-Semaphorin 4D Antibody , hFc Tag with the EC50 of 9.3ng/ml determined by ELISA.
