Human PLAU/uPA Protein (active form) Recombinant
Category: Receptors$202.00 – $675.00
Description
Accession
P00749
Source
DNA sequence encoding Human PLAU/uPA (active form) including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human PLAU/uPA (active form) is a protein consisting of amino acid residues [Ser21-Leu431] ,The protein has a predicted MW of 47.5 kDa. Due to protein lysis and glycosylation, the protein migrates to 24-26 kDa(long chain A), 35-38 kDa(chain B) and 52-60 kDa(long chain A&chain B) based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human PLAU/uPA (active form) was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Biological Process
Biological Process
Molecular function
Methods
Human PLAU/uPA Protein (active form) Tris-Bis PAGE
- Human PLAU (activated by trypsin) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human PLAU/uPA Protein (active form) SEC-HPLC
- The purity of Human PLAU (activated by trypsin) is greater than 95% as determined by SEC-HPLC.
Human PLAU/uPA Protein (active form) ELISA Data
- Immobilized Human PLAU, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human uPAR, hFc Tag with the EC50 of 10.3ng/ml determined by ELISA.
Human PLAU/uPA Protein (active form) SPR Data
- Mouse uPAR isoform 1, His Tag immobilized on CM5 Chip can bind Human PLAU, His Tag with an affinity constant of 5.87 nM as determined in SPR assay (Biacore T200).
