Description
Accession
P16234
Source
DNA sequence encoding Human PDGF R alpha/PDGFRA including a C-His-Avi tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human PDGF R alpha/PDGFRA is a protein consisting of amino acid residues [Gln24-Glu524] ,The protein has a predicted MW of 59.2 kDa. Due to glycosylation, the protein migrates to 70-83 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human PDGF R alpha/PDGFRA was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
A8T7D5
Interactor
Interactor
Interactor
Biological Process
Biological Process
Molecular function
Molecular function
Molecular function
Methods
Human PDGF R alpha/PDGFRA Protein Tris-Bis PAGE
- Human PDGF R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human PDGF R alpha/PDGFRA Protein SEC-HPLC
- The purity of Human PDGF R alpha is greater than 95% as determined by SEC-HPLC.
Human PDGF R alpha/PDGFRA Protein ELISA Data
- Immobilized Human PDGF R alpha, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-PDGF R alpha Antibody, hFc Tag with the EC50 of 11.0ng/ml determined by ELISA.
