Description
Accession
Q9BQ51
Source
DNA sequence encoding Human PD-L2/B7-DC including a C-His-Avi tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human PD-L2/B7-DC is a protein consisting of amino acid residues [Leu20-Thr220] ,The protein has a predicted MW of 25.3 kDa. Due to glycosylation, the protein migrates to 40-52 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human PD-L2/B7-DC was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Molecular function
Methods
Human PD-L2/B7-DC Protein Tris-Bis PAGE
- Human PD-L2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human PD-L2/B7-DC Protein SEC-HPLC
- The purity of Human PD-L2 is greater than 95% as determined by SEC-HPLC.
Human PD-L2/B7-DC Protein SPR Data
- Human PD-1, hFc Tag captured on CM5 Chip via Protein A can bind Human PD-L2, His Tag with an affinity constant of 0.296 μM as determined in SPR assay (Biacore T200).
