Description
Accession
Q15116
Source
DNA sequence encoding Human PD-1/PDCD1 including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human PD-1/PDCD1 is a protein consisting of amino acid residues [Leu25-Gln167] ,The protein has a predicted MW of 17 kDa. Due to glycosylation, the protein migrates to 38-50 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human PD-1/PDCD1 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Methods
Human PD-1/PDCD1 Protein Tris-Bis PAGE
- Human PD-1 on Tris-Bis PAGE under reduced. The purity is greater than 95%.
Human PD-1/PDCD1 Protein SEC-HPLC
- The purity of Human PD-1 is greater than 95% as determined by SEC-HPLC.
Human PD-1/PDCD1 Protein ELISA Data
- Immobilized Human PD-1, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-PD-1 Antibody, hFc Tag with the EC50 of 9.6ng/ml determined by ELISA.
