Description
Accession
Q15116
Source
DNA sequence encoding Human PD-1/PDCD1 including a C-hFc tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human PD-1/PDCD1 is a protein consisting of amino acid residues [Leu25-Gln167] ,The protein has a predicted MW of 42.6 kDa. Due to glycosylation, the protein migrates to 65-72 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human PD-1/PDCD1 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Methods
Human PD-1/PDCD1 Protein Tris-Bis PAGE
- Human PD-1 on Tris-Bis PAGE under reduced. The purity is greater than 95%.
Human PD-1/PDCD1 Protein SEC-HPLC
- The purity of Human PD-1 is greater than 95% as determined by SEC-HPLC.
Human PD-1/PDCD1 Protein ELISA Data
- Immobilized Human PD-1, hFc Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Anti-PD-1 Ab., hFc Tag with the EC50 of 31.2ng/ml determined by ELISA.
Human PD-1/PDCD1 Protein ELISA Data
- Immobilized Human PD-1, hFc Tag at 1μg/ml (100ul/Well). Dose response curve for Human PD-L1, mFc Tag with the EC50 of 0.1μg/ml determined by ELISA.
Human PD-1/PDCD1 Protein 37℃ Stability Test
- Immobilized Human PD-1, hFc Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Anti-PD-1 Ab., hFc Tag with the EC50 of 31.2/30.0/30.7/33.4ng/ml determined by ELISA.
Human PD-1/PDCD1 Protein SPR Data
- Human PD-1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human PD-L2, His-Avi Tag with an affinity constant of 0.344 μM as determined in SPR assay (Biacore T200).
