Description
Accession
P16109
Source
DNA sequence encoding Human P-Selectin/CD62P including a C-His-Avi tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human P-Selectin/CD62P is a protein consisting of amino acid residues [Trp42-Ala771] ,The protein has a predicted MW of 82.9 kDa. Due to glycosylation,the protein migrates to 100-120 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human P-Selectin/CD62P was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Biological Process
Methods
Human P-Selectin/CD62P Protein Tris-Bis PAGE
- Human P-Selectin/CD62P on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human P-Selectin/CD62P Protein SEC-HPLC
- The purity of Human P-Selectin/CD62P is greater than 95% as determined by SEC-HPLC.
Human P-Selectin/CD62P Protein ELISA Data
- Immobilized Human P-Selectin, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-P-Selectin Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA.
