Description
Accession
Q9UM47
Source
DNA sequence encoding Human Notch 3 including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human Notch 3 is a protein consisting of amino acid residues [Ala1378-Ser1640] ,The protein has a predicted MW of 30.18 kDa. Due to furin-like protease cleavage and glycosylation, the protein migrates to 27-30 kDa and 10-12 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human Notch 3 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Human Notch 3 Protein Tris-Bis PAGE
- Human Notch 3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human Notch 3 Protein SEC-HPLC
- The purity of Human Notch 3 is greater than 95% as determined by SEC-HPLC.
Human Notch 3 Protein ELISA Data
- Immobilized Human Notch 3, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Notch 3 Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA.
