Description
Accession
AAH52582
Source
DNA sequence encoding Human NKp30/NCR3/CD337 including a C-His-Avi tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human NKp30/NCR3/CD337 is a protein consisting of amino acid residues [Leu19-Thr138] ,The protein has a predicted MW of 20 kDa. Due to glycosylation, the protein migrates to 26-40 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human NKp30/NCR3/CD337 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Human NKp30/NCR3/CD337 Protein Tris-Bis PAGE
- Human NKp30 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
Human NKp30/NCR3/CD337 Protein SEC-HPLC
- The purity of Human NKp30 is greater than 95% as determined by SEC-HPLC.
Human NKp30/NCR3/CD337 Protein ELISA Data
- Immobilized Human NKp30, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-NKp30 Antibody, hFc Tag with the EC50 of 7.8ng/ml determined by ELISA.
Human NKp30/NCR3/CD337 Protein SPR Data
- Human B7-H6, hFc Tag captured on CM5 Chip via Protein A can bind Human NKp30, His Tag with an affinity constant of 0.292 μM as determined in SPR assay (Biacore T200).
