Description
Accession
P26718
Source
DNA sequence encoding Human NKG2D/CD314 including a N-His-Avi tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human NKG2D/CD314 is a protein consisting of amino acid residues [Phe78-Val216] ,The protein has a predicted MW of 19.0 kDa. Due to glycosylation, the protein migrates to 36-38 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human NKG2D/CD314 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Biological Process
Biological Process
Molecular function
Methods
Human NKG2D/CD314 Protein Tris-Bis PAGE
- Human NKG2D on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human NKG2D/CD314 Protein ELISA Data
- Immobilized Human NKG2D, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-NKG2D Antibody, hFc Tag with the EC50 of 12.2ng/ml determined by ELISA.
Human NKG2D/CD314 Protein ELISA Data
- Immobilized Human NKG2D, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human MICA, hFc Tag with the EC50 of 33.4ng/ml determined by ELISA.
Human NKG2D/CD314 Protein ELISA Data
- Immobilized Human NKG2D, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human ULBP-6, hFc Tag with the EC50 of 12.9ng/ml determined by ELISA.
Human NKG2D/CD314 Protein SPR Data
- Human MICB, hFc Tag captured on CM5 Chip via Protein A can bind Human NKG2D, His Tag with an affinity constant of 95.15 nM as determined in SPR assay (Biacore T200).
