Description
Accession
P26718
Source
DNA sequence encoding Human NKG2D/CD314 including a N-hFc-Flag tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Human NKG2D/CD314 is a protein consisting of amino acid residues [Phe78-Val216] ,The protein has a predicted MW of 43.4 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human NKG2D/CD314 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Biological Process
Biological Process
Molecular function
Methods
Human NKG2D/CD314 Protein Tris-Bis PAGE
- Human NKG2D on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Human NKG2D/CD314 Protein SEC-HPLC
- The purity of Human NKG2D is greater than 95% as determined by SEC-HPLC.
Human NKG2D/CD314 Protein ELISA Data
- Immobilized Human MICA, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.14μg/ml determined by ELISA.
Human NKG2D/CD314 Protein ELISA Data
- Immobilized Human ULBP-2, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 2.8μg/ml determined by ELISA.
Human NKG2D/CD314 Protein Blocking Data
- Serial dilutions of Anti-NKG2D Antibody, hFc Tag were added into Biotinylated Human ULBP-2, His Tag : Human NKG2D, hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 45.2ng/ml.
